rabbit polyclonal anti phospho eef2  (Bioss)

Journal: iScience

Article Title: Synaptotagmin-7 deficit causes insulin hypoactivity and contributes to behavioral alterations in mice

doi: 10.1016/j.isci.2025.112354

Figure Lengend Snippet: Signaling pathways involved in the genesis of mood cycling (A) Schematic showing comparative analysis of hippocampal gene expression profiles between manic and depressive Syt7 KO mice. GeneM, comparison between the WT mice and the manic Syt7 KO mice. GeneD, comparison between the WT mice and the depressive Syt7 KO mice. (B and C) Heatmap (B) and distribution (C) of genes differentially expressed between GeneM and GeneD. (D) Expression of representative BD-related genes in manic and depressive Syt7 KO mice. (E) Representative significantly enriched KEGG pathways showing bi-directional alterations in the manic and depressive episodes in Syt7 KO mice. (F and G) Schematic (F) and heatmap (G) showing the comparative analysis of transcripts bi-directionally regulated in drug-induced/Syt7 deficiency-induced manic and depressive mice. GeneR, comparison between the WT mice with and without the Ro25 treatment in the dark phase. GeneB, comparison between the WT mice with and without the BMS-536924 treatment in the light phase. (H) Representative significantly enriched KEGG pathways showing bi-directional alterations between the drug-induced/Syt7-deficiency-induced manic and depressive mice. (I) Mapping of representative KEGG pathways for network analysis. (J) Bi-directional regulation of representative genes within featured KEGG pathways in mice with drug-induced/Syt7-deficiency-induced mania or depression. (K and L) Immunoblots (left) and quantitative analysis (right) of mammalian targets of rapamycin (mTOR) (K) and eukaryotic elongation factor 2 (eEF2) (L) phosphorylation in the hippocampus of WT and Syt7 KO mice. n = 3. Student’s t test; ∗ p < 0.05; ∗∗ p < 0.001; error bars, SEM.

Article Snippet: Rabbit polyclonal anti- p -eEF2 , Cell Signaling , Cat# 2331S; RRID: N/A.

Techniques: Protein-Protein interactions, Gene Expression, Comparison, Expressing, Western Blot, Phospho-proteomics

Journal: iScience

Article Title: Synaptotagmin-7 deficit causes insulin hypoactivity and contributes to behavioral alterations in mice

doi: 10.1016/j.isci.2025.112354

Figure Lengend Snippet: Signaling pathways involved in the genesis of mood cycling (A) Schematic showing comparative analysis of hippocampal gene expression profiles between manic and depressive Syt7 KO mice. GeneM, comparison between the WT mice and the manic Syt7 KO mice. GeneD, comparison between the WT mice and the depressive Syt7 KO mice. (B and C) Heatmap (B) and distribution (C) of genes differentially expressed between GeneM and GeneD. (D) Expression of representative BD-related genes in manic and depressive Syt7 KO mice. (E) Representative significantly enriched KEGG pathways showing bi-directional alterations in the manic and depressive episodes in Syt7 KO mice. (F and G) Schematic (F) and heatmap (G) showing the comparative analysis of transcripts bi-directionally regulated in drug-induced/Syt7 deficiency-induced manic and depressive mice. GeneR, comparison between the WT mice with and without the Ro25 treatment in the dark phase. GeneB, comparison between the WT mice with and without the BMS-536924 treatment in the light phase. (H) Representative significantly enriched KEGG pathways showing bi-directional alterations between the drug-induced/Syt7-deficiency-induced manic and depressive mice. (I) Mapping of representative KEGG pathways for network analysis. (J) Bi-directional regulation of representative genes within featured KEGG pathways in mice with drug-induced/Syt7-deficiency-induced mania or depression. (K and L) Immunoblots (left) and quantitative analysis (right) of mammalian targets of rapamycin (mTOR) (K) and eukaryotic elongation factor 2 (eEF2) (L) phosphorylation in the hippocampus of WT and Syt7 KO mice. n = 3. Student’s t test; ∗ p < 0.05; ∗∗ p < 0.001; error bars, SEM.

Article Snippet: Primary antibodies were as follows: mouse monoclonal anti-Actin antibody (1:5000, Abcam, #ab6276), rabbit polyclonal anti-mTOR antibody (1:500, Cell Signaling, #2972), rabbit polyclonal anti- p -mTOR antibody (1:500, Cell Signaling, #2971), rabbit polyclonal anti-eEF2 antibody (1:1000, Cell Signaling, #2332S), rabbit polyclonal anti- p -eEF2 antibody (1:1000, Cell Signaling, #2331S).

Techniques: Protein-Protein interactions, Gene Expression, Comparison, Expressing, Western Blot, Phospho-proteomics

Journal: iScience

Article Title: Synaptotagmin-7 deficit causes insulin hypoactivity and contributes to behavioral alterations in mice

doi: 10.1016/j.isci.2025.112354

Figure Lengend Snippet: Signaling pathways involved in the genesis of mood cycling (A) Schematic showing comparative analysis of hippocampal gene expression profiles between manic and depressive Syt7 KO mice. GeneM, comparison between the WT mice and the manic Syt7 KO mice. GeneD, comparison between the WT mice and the depressive Syt7 KO mice. (B and C) Heatmap (B) and distribution (C) of genes differentially expressed between GeneM and GeneD. (D) Expression of representative BD-related genes in manic and depressive Syt7 KO mice. (E) Representative significantly enriched KEGG pathways showing bi-directional alterations in the manic and depressive episodes in Syt7 KO mice. (F and G) Schematic (F) and heatmap (G) showing the comparative analysis of transcripts bi-directionally regulated in drug-induced/Syt7 deficiency-induced manic and depressive mice. GeneR, comparison between the WT mice with and without the Ro25 treatment in the dark phase. GeneB, comparison between the WT mice with and without the BMS-536924 treatment in the light phase. (H) Representative significantly enriched KEGG pathways showing bi-directional alterations between the drug-induced/Syt7-deficiency-induced manic and depressive mice. (I) Mapping of representative KEGG pathways for network analysis. (J) Bi-directional regulation of representative genes within featured KEGG pathways in mice with drug-induced/Syt7-deficiency-induced mania or depression. (K and L) Immunoblots (left) and quantitative analysis (right) of mammalian targets of rapamycin (mTOR) (K) and eukaryotic elongation factor 2 (eEF2) (L) phosphorylation in the hippocampus of WT and Syt7 KO mice. n = 3. Student’s t test; ∗ p < 0.05; ∗∗ p < 0.001; error bars, SEM.

Article Snippet: Primary antibodies were as follows: mouse monoclonal anti-Actin antibody (1:5000, Abcam, #ab6276), rabbit polyclonal anti-mTOR antibody (1:500, Cell Signaling, #2972), rabbit polyclonal anti- p -mTOR antibody (1:500, Cell Signaling, #2971), rabbit polyclonal anti-eEF2 antibody (1:1000, Cell Signaling, #2332S), rabbit polyclonal anti- p -eEF2 antibody (1:1000, Cell Signaling, #2331S).

Techniques: Protein-Protein interactions, Gene Expression, Comparison, Expressing, Western Blot, Phospho-proteomics